Vector = the term used for an agent that can carry something
from one organism to another.
type of Vector often used to transfer genes is a Plasmid.
are small circular pieces of DNA found in most bacteria. They
are completely separate from the main bacterial DNA and lie
freely in the cytoplasm. They are not however useless and
many contain genes which make bacteria resistant to a particular
often use this antibiotic resistance to ensure that their
required gene has been inserted into a plasmid to make the
new recombinant DNA molecule. Using them as Genetic markers.
is carried out as follows: -
recombinant plasmid will no longer be able to express Ampicillin
not all the plasmids will take up the Donor DNA and some will
just re-glue themselves back together.
do we identify which plasmids contain the gene we require
and which have not?