a Genomic Library of DNA
to find a needle in
a haystack ,
or how to get the 10,000 base pairs you need out of the 10
billion base pairs you don't!
do you cut the gene you need out of all the genetic material
available in a cells nucleus?
In this method we take the shotgun approach,
blasting the whole nucleus apart and searching through all
the fragments in the hope of finding our required DNA piece.
Firstly cut up all of the Donors DNA with special "enzyme
to make lots of different sized fragments or strands of DNA.
Endonucleases are the "scissors" used to cut up
the DNA. These enzymes are made naturally by bacteria so they
can eat and destroy any invading viral DNA.
restriction enzymes cut the DNA at specific base sequences
can either cut to make Blunt ends :-
Sticky ends :-
Then search through all the fragments to Find
the piece you want